TBPL1 recombinant protein

TBPL1, 1-186aa, Human, His tag, E Coli

Cat. Num. AAA205112

• Gene Names: TBPL1; TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TBPL1; 1-186aa; Human; His tag; E Coli; TATA box-binding protein-like protein 1; MGC8389; MGC9620; STuD; TLF; TLP; TRF2; TBPL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL
• Sequence Length: 186

• Applicable Applications for TBPL1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TBPL1 recombinant protein

TBPL1, also known as STuD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TBPL1 recombinant protein

Epigenomics

References

Ohbayashi T., et al. (1999) Biochem. Biophys. Res. Commun. 255:137-142; Rabenstein M.D., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:4791-4796

NCBI and Uniprot Product Information

• NCBI GI #: 4759234
• NCBI GeneID: 9519
• NCBI Accession #: NP_004856
• NCBI GenBank Nucleotide #: NM_004865.3
• UniProt Accession #: P62380; O95753; Q9BWD5; Q9Z2Z0; A8K8F5
• Molecular Weight: 23.3kDa (209aa), confirmed by MALDI-TOF
• NCBI Official Full Name: TATA box-binding protein-like protein 1
• NCBI Official Synonym Full Names: TATA-box binding protein like 1
• NCBI Official Symbol: TBPL1
• NCBI Official Synonym Symbols: TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
• NCBI Protein Information: TATA box-binding protein-like protein 1
• UniProt Protein Name: TATA box-binding protein-like protein 1
• Protein Family: TATA box-binding protein-like protein
• UniProt Gene Name: TBPL1
• UniProt Synonym Gene Names: TLF; TLP; TLP21; TRF2; TRP; TBP-like protein 1; STUD; TBP-related factor 2

NCBI Description

This gene encodes a member of the TATA box-binding protein family. TATA box-binding proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA box and initiates transcription from TATA-less promoters. This gene plays a critical role in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Nov 2011]

Uniprot Description

Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.