SDS-Page
TATA Binding Protein Recombinant Protein | TBP recombinant protein
Recombinant TATA Binding Protein (TBP)
Gene Names
Tbp; CG9874; DmelCG9874; dtbp; dTbp; dTBP; dTFIID; RBP; TBP; TBP38; TfIID; TFIID; TFIIDtau
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
TATA Binding Protein; Recombinant TATA Binding Protein (TBP); TBP recombinant protein
Host
E.coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
PPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE AFENIYPILK GFRKTT
Applicable Applications for TBP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Pro8~Thr316
Tags
N-terminal His Tag
Subcellular Location
Nucleus
Traits
Freeze-dried powder
Predicted isoelectric point
10.0
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 37.6kDa
Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
TATA binding protein
NCBI Official Synonym Full Names
TATA binding protein
NCBI Official Symbol
Tbp
NCBI Official Synonym Symbols
CG9874; DmelCG9874; dtbp; dTbp; dTBP; dTFIID; RBP; TBP; TBP38; TfIID; TFIID; TFIIDtau
NCBI Protein Information
CG9874 gene product from transcript CG9874-RA
UniProt Protein Name
TATA-box-binding protein
Protein Family
UniProt Gene Name
Tbp
UniProt Synonym Gene Names
BTF1; TFIID
UniProt Entry Name
TBP_DROME
Uniprot Description
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Research Articles on TBP
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Product Notes
The TBP tbp (Catalog #AAA2031655) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TATA Binding Protein can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB) . Researchers should empirically determine the suitability of the TBP tbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE AFENIYPILK GFRKTT. It is sometimes possible for the material contained within the vial of "TATA Binding Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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