Rabbit anti-Human PRMT7 Polyclonal Antibody | anti-PRMT7 antibody

PRMT7 Antibody (N-term)

Cat. Num. AAA289800

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PRMT7, Antibody; PRMT7 Antibody (N-term); Protein arginine N-methyltransferase 7; 211-; Histone-arginine N-methyltransferase PRMT7; [Myelin basic protein]-arginine N-methyltransferase PRMT7; PRMT7; KIAA1933; anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PRMT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRMT7.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 642

• Applicable Applications for anti-PRMT7 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of PRMT7 (arrow) using rabbit polyclonal PRMT7 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).)

Related Product Information for anti-PRMT7 antibody

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

References

Lee,J.H., et al. J. Biol. Chem. 280 (5), 3656-3664 (2005)
Miranda,T.B., et al. J. Biol. Chem. 279 (22), 22902-22907 (2004)
Frankel A., et al. J. Biol. Chem. 277:3537-3543(2002).
Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003).
Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001).
Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999).
Gilbreth, M., et al., PNAS 95(25):14781-14786 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 296317343
• NCBI GeneID: 54496
• NCBI Accession #: NP_001171753.1; NP_061896.1
• NCBI GenBank Nucleotide #: NM_001184824.1
• UniProt Accession #: Q9NVM4; Q96PV5; Q9H9L0; B3KPR0; B3KUG9; B4E379
• Molecular Weight: 78459
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 2
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Protein Information: protein arginine N-methyltransferase 7
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN