Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myotubularin-related protein 3 (MTMR3) Standard Curve.)
Rat Myotubularin-Related Protein 3 (MTMR3) ELISA Kit | MTMR3 elisa kit
Rat Myotubularin-Related Protein 3 (MTMR3) ELISA Kit
Reactivity
Rat
Synonyms
Myotubularin-Related Protein 3 (MTMR3); Rat Myotubularin-Related Protein 3 (MTMR3) ELISA Kit; MTMR3; hCG_2011013; FLJ32333; FYVE-DSP1; KIAA0371; ZFYVE10; FYVE (Fab1 YGLO23 Vsp27 EEA1 domain) dual-specificity protein phosphatase; OTTHUMP00000199667; OTTHUMP00000199668; myotubularin-related protein 3; zi; MTMR3 elisa kit
Reactivity
Rat
Specificity
Rat Myotubularin-related protein 3 (MTMR3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MTMR3. No significant cross-reactivity or interference between Rat MTMR3 and analogues was observed.
Sequence Length
1194
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myotubularin-related protein 3 (MTMR3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myotubularin-related protein 3 (MTMR3) Standard Curve.)
Related Product Information for MTMR3 elisa kit
Description: This Rat Myotubularin-related protein 3 (MTMR3) ELISA Kit employs a two-site sandwich ELISA to quantitate MTMR3 in samples. An antibody specific for MTMR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTMR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTMR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTMR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MTMR3 encodes a member of the myotubularin dual specificity protein phosphatase gene family. The encoded protein is structurally similar to myotubularin but in addition contains a FYVE domain and an N-terminal PH-GRAM domain. The protein can self-associate and also form heteromers with another myotubularin related protein. The protein binds to phosphoinositide lipids through the PH-GRAM domain, and can hydrolyze phosphatidylinositol(3)-phosphate and phosphatidylinositol(3,5)-biphosphate in vitro. The encoded protein has been observed to have a perinuclear, possibly membrane-bound, distribution in cells, but it has also been found free in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for MTMR3.
Background: MTMR3 encodes a member of the myotubularin dual specificity protein phosphatase gene family. The encoded protein is structurally similar to myotubularin but in addition contains a FYVE domain and an N-terminal PH-GRAM domain. The protein can self-associate and also form heteromers with another myotubularin related protein. The protein binds to phosphoinositide lipids through the PH-GRAM domain, and can hydrolyze phosphatidylinositol(3)-phosphate and phosphatidylinositol(3,5)-biphosphate in vitro. The encoded protein has been observed to have a perinuclear, possibly membrane-bound, distribution in cells, but it has also been found free in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for MTMR3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myotubularin-related protein 3
NCBI Official Synonym Full Names
myotubularin related protein 3
NCBI Official Symbol
Mtmr3
NCBI Protein Information
myotubularin-related protein 3
UniProt Protein Name
Myotubularin-related protein 3
UniProt Gene Name
Mtmr3
UniProt Entry Name
MTMR3_RAT
Similar Products
Product Notes
The Rat MTMR3 mtmr3 (Catalog #AAA9712217) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712217 ELISA Kit recognizes Rat MTMR3. It is sometimes possible for the material contained within the vial of "Myotubularin-Related Protein 3 (MTMR3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
