Human TATA-box-binding protein ELISA Kit | TBP elisa kit

Human TATA-box-binding protein ELISA Kit

Cat. Num. AAA2882420

• Gene Names: TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
• Reactivity: Human
• Synonyms: TATA-box-binding protein; Human TATA-box-binding protein ELISA Kit; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; TBP; GTF2D1; TF2D; TFIID; TBP elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human TATA-box-binding protein
• Sequence Length: 319

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: < 0.169 ng/mL
• Intra-assay Precision: <=4.8%
• Inter-assay Precision: <=7.5%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TBP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 285026520
• NCBI GeneID: 6908
• NCBI Accession #: NP_001165556.1
• NCBI GenBank Nucleotide #: NM_001172085.1
• UniProt Accession #: P20226; Q16845; Q6IBM6; Q9UC02; B4E3B3; F5H869
• Molecular Weight: 35,657 Da
• NCBI Official Full Name: TATA-box-binding protein isoform 2
• NCBI Official Synonym Full Names: TATA-box binding protein
• NCBI Official Symbol: TBP
• NCBI Official Synonym Symbols: HDL4; GTF2D; SCA17; TFIID; GTF2D1
• NCBI Protein Information: TATA-box-binding protein
• UniProt Protein Name: TATA-box-binding protein
• Protein Family: TATA-box-binding protein
• UniProt Gene Name: TBP
• UniProt Synonym Gene Names: GTF2D1; TF2D; TFIID
• UniProt Entry Name: TBP_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.