Testing Data
Rabbit USP3 Polyclonal Antibody | anti-USP3 antibody
USP3 Antibody
Gene Names
USP3; UBP; SIH003
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
USP3; Polyclonal Antibody; USP3 Antibody; MGC129878; MGC129879; SIH003; UBP; anti-USP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug of antibody in 100 ul PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Appearance: Colorless liquid
Appearance: Colorless liquid
Sequence Length
476
Applicable Applications for anti-USP3 antibody
Western Blot (WB)
Application Notes
Western blot: 1:500 - 1:1000 dilution. However, the optimal conditions should be determined individually.
Immunogen
Fusion protein of human USP3
Positive Control
Western Blot: Various cell lysates
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-USP3 antibody
Polyclonal antibody to detect USP3 (a DUB enzyme) in human, mouse and rat samples.
Background: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP3 (ubiquitin specific peptidase3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Background: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP3 (ubiquitin specific peptidase3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Product Categories/Family for anti-USP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,743 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 3 isoform 2
NCBI Official Synonym Full Names
ubiquitin specific peptidase 3
NCBI Official Symbol
USP3
NCBI Official Synonym Symbols
UBP; SIH003
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 3
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 3
UniProt Gene Name
USP3
UniProt Entry Name
UBP3_HUMAN
Uniprot Description
USP3: Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. Belongs to the peptidase C19 family. USP3 subfamily.
Protein type: Protease; Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12
Chromosomal Location of Human Ortholog: 15q22.3
Cellular Component: nuclear chromatin
Molecular Function: histone binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; chromatin binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; DNA repair; histone deubiquitination
Research Articles on USP3
Similar Products
Product Notes
The USP3 usp3 (Catalog #AAA841298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot: 1:500 - 1:1000 dilution. However, the optimal conditions should be determined individually. Researchers should empirically determine the suitability of the USP3 usp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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