Typical Testing Data/Standard Curve (for reference only)
Mouse TANK Binding Kinase 1 ELISA Kit | TBK1 elisa kit
Mouse TBK1 (TANK Binding Kinase 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TBK1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TBK1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TBK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TBK1.You can calculate the concentration of TBK1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
Uniprot Description
TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Other group; IKK family
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: cytoplasm; cytosol; endosome membrane
Molecular Function: ATP binding; nucleic acid binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; interferon type I production; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to virus; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; viral reproduction
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4
Research Articles on TBK1
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Product Notes
The Mouse TBK1 tbk1 (Catalog #AAA2533500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533500 ELISA Kit recognizes Mouse TBK1. It is sometimes possible for the material contained within the vial of "TANK Binding Kinase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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