Mouse Serine/threonine-protein kinase TBK1 ELISA Kit | Tbk1 elisa kit

Mouse Serine/threonine-protein kinase TBK1 ELISA Kit

Cat. Num. AAA761047

• Gene Names: TBK1; NAK; T2K; FTDALS4
• Reactivity: Mouse
• Synonyms: Serine/threonine-protein kinase TBK1; Mouse Serine/threonine-protein kinase TBK1 ELISA Kit; Tbk1/T2K/TANK-binding kinase 1; Tbk1 elisa kit

• Reactivity: Mouse
• Sequence Length: 729

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Tbk1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Tbk1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Tbk1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Tbk1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Tbk1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7019547
• NCBI GeneID: 29110
• NCBI Accession #: NP_037386.1
• NCBI GenBank Nucleotide #: NM_013254.3
• Molecular Weight: 83,642 Da
• NCBI Official Full Name: serine/threonine-protein kinase TBK1
• NCBI Official Synonym Full Names: TANK-binding kinase 1
• NCBI Official Symbol: TBK1
• NCBI Official Synonym Symbols: NAK; T2K; FTDALS4
• NCBI Protein Information: serine/threonine-protein kinase TBK1
• UniProt Protein Name: Serine/threonine-protein kinase TBK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: TBK1
• UniProt Synonym Gene Names: NAK
• UniProt Entry Name: TBK1_HUMAN

NCBI Description

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]

Uniprot Description

TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; IKK family

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm; cytosol; endosome membrane

Molecular Function: ATP binding; nucleic acid binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; interferon type I production; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to virus; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; viral reproduction

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4