Tubulin cofactor A Recombinant Protein | TBCA recombinant protein

Tubulin cofactor A Protein, Human, Recombinant

Cat. Num. AAA8121582

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Tubulin cofactor A; Tubulin cofactor A Protein; Human; Recombinant; Human Tubulin folding cofactor A/TBCA Protein; tubulin folding cofactor A; TBCA recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala108

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human TBCA (O75347)(Met1-Ala108) was expressed, with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TBCA recombinant protein

Background: Tubulin folding cofactor A belongs to the TBCA family. It is one of four proteins (cofactors A, D, E, and C) involved in the early step of the tubulin folding pathway. These proteins can fold intermediates and finally lead to correctly folded beta-tubulin. It is believed that tubulin folding cofactors A and D play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Tubulin folding cofactor E binds to the cofactor D/beta-tubulin complex; interaction with tubulin folding cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

References

Strausberg RL, et al. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.Irwin DM, et al. (2003) Molecular evolution of vertebrate goose-type lysozyme genes. J Mol Evol. 56(2):234-42.Sklar P, et al. (2011) Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4. Nat Genet. 43(10):977-83.

NCBI and Uniprot Product Information

• NCBI GI #: 663071149
• NCBI GeneID: 6902
• NCBI Accession #: NP_001284667.1
• NCBI GenBank Nucleotide #: NM_001297738.1
• UniProt Accession #: O75347; B4DT30
• Molecular Weight: 15,792 Da
• NCBI Official Full Name: tubulin-specific chaperone A isoform 1
• NCBI Official Synonym Full Names: tubulin folding cofactor A
• NCBI Official Symbol: TBCA
• NCBI Protein Information: tubulin-specific chaperone A; CFA; TCP1-chaperonin cofactor A; tubulin-folding cofactor A
• UniProt Protein Name: Tubulin-specific chaperone A
• UniProt Gene Name: TBCA
• UniProt Synonym Gene Names: CFA
• UniProt Entry Name: TBCA_HUMAN

NCBI Description

The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

TBCA: Tubulin-folding protein; involved in the early step of the tubulin folding pathway. Belongs to the TBCA family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: microtubule cytoskeleton; microtubule; cytoplasm; nucleolus

Molecular Function: chaperone binding; unfolded protein binding

Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; post-chaperonin tubulin folding pathway