Phospholipid Scramblase 1 Recombinant Protein | PLSCR1 recombinant protein

Recombinant Phospholipid Scramblase 1 (PLSCR1)

Cat. Num. AAA2030655

• Gene Names: PLSCR1; MMTRA1B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Phospholipid Scramblase 1; Recombinant Phospholipid Scramblase 1 (PLSCR1); PLSCR1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MDKQNSQMNA SHPETNLPVG YPPQYPPTAF QGPPGYSGYP GPQVSYPPPP AGHSGPGPAG FPVPNQPVYN QPVYNQPVGA AGVPWMPAPQ PPLNCPPGLE YLSQIDQILI HQQIELLEVL TGFETNNKYE IKNSFGQRVY FAAEDTDCCT RNCCGPSRPF TLRIIDNMGQ EVITLERPLR CSSCCCPCCL QEIEIQAPPG VPIGYVIQTW HPCLPKFTIQ NEKREDVLKI SGPCVVCSCC GDVDFEIKSL DEQCVVGKIS KHWTGILREA FTDADNFGIQ FPLDLDVKMKAVMIGACFLI DFMFFESTGS QEQKSGVW
• Sequence Length: 318

• Applicable Applications for PLSCR1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 38.7kDa
• Accurate Molecular Mass (KD): 39kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Trp318
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 10863877
• NCBI GeneID: 5359
• NCBI Accession #: NP_066928.1
• NCBI GenBank Nucleotide #: NM_021105.2
• Molecular Weight: 26,825 Da
• NCBI Official Full Name: phospholipid scramblase 1
• NCBI Official Synonym Full Names: phospholipid scramblase 1
• NCBI Official Symbol: PLSCR1
• NCBI Official Synonym Symbols: MMTRA1B
• NCBI Protein Information: phospholipid scramblase 1
• UniProt Protein Name: Phospholipid scramblase 1
• Protein Family: Phospholipid scramblase
• UniProt Gene Name: PLSCR1
• UniProt Synonym Gene Names: PL scramblase 1
• UniProt Entry Name: PLS1_HUMAN

Uniprot Description

PLSCR1: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family.

Protein type: Calcium-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: Golgi apparatus; extracellular matrix; membrane; integral to plasma membrane; plasma membrane; nucleolus; cytosol; nucleus; lipid raft

Molecular Function: phospholipid scramblase activity; protein binding; enzyme binding; DNA binding; calcium ion binding; CD4 receptor binding; SH3 domain binding; epidermal growth factor receptor binding

Biological Process: platelet activation; negative regulation of viral genome replication; apoptosis; positive regulation of innate immune response; acute-phase response; positive regulation of transcription from RNA polymerase II promoter; phospholipid scrambling; defense response to virus; regulation of mast cell activation; phosphatidylserine biosynthetic process