TAF10 recombinant protein

TAF10, 84-218aa, Human, His tag, E Coli

Cat. Num. AAA205173

• Gene Names: TAF10; TAF2A; TAF2H; TAFII30
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TAF10; 84-218aa; Human; His tag; E Coli; transcription initiation factor TFIID subunit 10; TAF2A; TAF2H; TAFII30; TAF10 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT
• Sequence Length: 218

• Applicable Applications for TAF10 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TAF10 recombinant protein

TAF10 is one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. Recombinant human TAF10 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TAF10 recombinant protein

Epigenomics

References

Brand M., et al (1999). J. Biol. Chem. 274:18285-18289; Gangloff Y.G., et al (2001). Mol. Cell. Biol. 21:5109-5121;

NCBI and Uniprot Product Information

• NCBI GI #: 5454106
• NCBI GeneID: 6881
• NCBI Accession #: NP_006275
• NCBI GenBank Nucleotide #: NM_006284.3
• UniProt Accession #: Q12962; O00703; Q13175; Q6FH13
• Molecular Weight: 16.9 kDa (158aa)
• NCBI Official Full Name: transcription initiation factor TFIID subunit 10
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 10
• NCBI Official Symbol: TAF10
• NCBI Official Synonym Symbols: TAF2A; TAF2H; TAFII30
• NCBI Protein Information: transcription initiation factor TFIID subunit 10
• UniProt Protein Name: Transcription initiation factor TFIID subunit 10
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF10
• UniProt Synonym Gene Names: TAF2A; TAF2H; TAFII30; TAF(II)30; TAFII-30; TAFII30

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008]

Uniprot Description

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.