Endonuclease V Recombinant Protein | denV recombinant protein
Recombinant Endonuclease V
Applications
Western Blot, ELISA
Purity
Greater than 95% by SDS-PAGE gel analyses
Synonyms
Endonuclease V; Recombinant Endonuclease V; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; T4 pyrimidine dimer glycosylase; T4-Pdg; denV recombinant protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE gel analyses
Form/Format
Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
Sequence Positions
1-138
Applicable Applications for denV recombinant protein
Western Blot (WB), ELISA (EIA)
Species
T4 phage
Tag
N-terminal His-IF2DI Tag
Reconstitution
Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
Preparation and Storage
Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,079 Da
NCBI Official Full Name
DenV endonuclease V, N-glycosylase UV repair enzyme
NCBI Official Symbol
denV
NCBI Protein Information
bifunctional N-glycosylase and DNA endonulcease for repair of pyrimidine dimers
UniProt Protein Name
Endonuclease V
Protein Family
UniProt Gene Name
T4-Pdg
UniProt Synonym Gene Names
T4-Pdg
Uniprot Description
Participates in the repair of UV-damaged DNA by excising pyrimidine dimers that are the major UV-lesions (PubMed:6254991). DNA glycosylase activity hydrolyzes the glycosylic bond of the 5' pyrimidine of the dimer (PubMed:6254991). This leaves apurinic/apyrimidic (AP) sites in the DNA. These AP sites are removed by the AP lyase activity which cleaves the intrapyrimidine phosphodiester bond (PubMed:6254991). Catalysis proceeds via a protonated imine covalent intermediate between the alpha-amino group of the N-terminal threonine residue and the C1' of the deoxyribose sugar of the 5' pyrimidine at the dimer site (PubMed:8347626) (PubMed:16916523).
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Product Notes
The denV t4-pdg (Catalog #AAA7614905) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-138. AAA Biotech's Endonuclease V can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the denV t4-pdg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endonuclease V, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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