Synaptotagmin-1 Recombinant Protein | SYT1 recombinant protein

Recombinant Human Synaptotagmin-1

Cat. Num. AAA9420719

• Gene Names: SYT1; P65; SYT; SVP65
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Synaptotagmin-1; Recombinant Human Synaptotagmin-1; Synaptotagmin I; SytIp65; SYT1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Cytoplasmic Domain, 99-416aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for SYT1 recombinant protein

May have a regulatory role in the mbrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

NCBI and Uniprot Product Information

• NCBI GI #: 209447070
• NCBI GeneID: 6857
• NCBI Accession #: NP_001129277.1
• NCBI GenBank Nucleotide #: NM_001135805.1
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 40.4kD
• NCBI Official Full Name: synaptotagmin-1 isoform 1
• NCBI Official Synonym Full Names: synaptotagmin 1
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65
• NCBI Protein Information: synaptotagmin-1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT; SytI

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Uniprot Description

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Protein type: Calcium-binding; Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q21.2

Cellular Component: clathrin coated vesicle membrane; neuron projection; plasma membrane; synaptic vesicle; synaptic vesicle membrane

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; low-density lipoprotein receptor binding; phosphatidylinositol binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; SNARE binding; syntaxin-1 binding

Biological Process: calcium ion-dependent exocytosis of neurotransmitter; detection of calcium ion; glutamate secretion; neurotransmitter secretion; positive regulation of synaptic transmission; protein homooligomerization; regulation of calcium ion-dependent exocytosis; regulation of exocytosis; regulation of synaptic transmission, glutamatergic; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion