Rabbit anti-Human, Mouse PALLD Polyclonal Antibody | anti-PALLD antibody

PALLD Antibody

Cat. Num. AAA9610048

• Gene Names: PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PALLD; Polyclonal Antibody; PALLD Antibody; CGI 151; CGI151; KIAA0992; MYN; Myoneurin; pa; Palladin cytoskeletal associated protein; Palld; PNCA1; Sarcoma antigen NY SAR 77; SIH002; Stx13bp1; anti-PALLD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PALLD antibody detects endogenous levels of total PALLD
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1123

• Applicable Applications for anti-PALLD antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human PALLD
• Subcellular Location: Cytoplasm, Cytoskeleton. Note: Localizes to stress fibers and Z disks. Cell Junction, Focal Adhesion. Cell Projection, Ruffle. Cell Projection, Lamellipodium.
• Tissue Specificity: Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines.
• Predicted Cross Reactivity: Zebrafish, Horse, Sheep, Rabbit, Dog
• Similarity: Zebrafish (88%), Horse (100%), Sheep (80%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PALLD using HepG2 whole cell lysates)

Related Product Information for anti-PALLD antibody

Function: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.
Subunit Structure: Interacts with EPS8 (By similarity). Interacts with LASP1 (By similarity). Interacts with VASP (By similarity). Interacts with ACTN (PubMed:15147863). Interacts with ARGBP2 (PubMed:16125169). Interacts with PFN1 (PubMed:16367745). Interacts with LPP (PubMed:17322171). Interacts with SPIN90 (PubMed:17537434). Interacts with SRC (PubMed:17537434). Interacts with EZR (PubMed:11598191). Interacts with RAI14 (By similarity).
Post-translational Modifications: Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines (By similarity). Phosphorylation at Ser-1118 by PKB/AKT1 modulates cytoskeletal organization and cell motility.
Similarity: Belongs to the myotilin/palladin family.

References

Wu D, Huang CJ, Jiao XF, Ding ZM, Zhang JY, Chen F, Wang YS, Li X, Huo LJ; Journal: Oncotarget. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.

NCBI and Uniprot Product Information

• NCBI GI #: 260656030
• NCBI GeneID: 23022
• NCBI Accession #: NP_001159580.1
• NCBI GenBank Nucleotide #: NM_001166108.1
• UniProt Accession #: Q8WX93; Q7L3E0; Q7Z3W0; Q86WE8; Q8N1M2; Q9UGA0; Q9UQF5; Q9Y2J6; Q9Y3E9; B3KTG2; B5MD56; B7ZMM5
• Molecular Weight: Observed: 151 kDa; Predicted: 151 kDa
• NCBI Official Full Name: palladin isoform 1
• NCBI Official Synonym Full Names: palladin, cytoskeletal associated protein
• NCBI Official Symbol: PALLD
• NCBI Official Synonym Symbols: MYN; PNCA1; CGI151; SIH002; CGI-151
• NCBI Protein Information: palladin
• UniProt Protein Name: Palladin
• Protein Family: Palladin
• UniProt Gene Name: PALLD
• UniProt Synonym Gene Names: KIAA0992

NCBI Description

This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.