SYT1 sirna

SYT1 siRNA (Rat)

Cat. Num. AAA8201638

• Gene Names: Syt1; P65
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYT1; SYT1 siRNA (Rat); Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SYT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for SYT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SYT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYT1 sirna

siRNA to inhibit SYT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148356226
• NCBI GeneID: 25716
• NCBI Accession #: NP_001028852.2
• NCBI GenBank Nucleotide #: NM_001033680.2
• UniProt Accession #: P21707; Q3S2E6; Q707P5
• Molecular Weight: 47,399 Da
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: Syt1
• NCBI Official Synonym Symbols: P65
• NCBI Protein Information: synaptotagmin-1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: Syt1
• UniProt Synonym Gene Names: SytI
• UniProt Entry Name: SYT1_RAT

NCBI Description

integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

Uniprot Description

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Protein type: Calcium-binding; Vesicle; Membrane protein, integral

Cellular Component: neuron projection; synaptic vesicle membrane; cell; integral to membrane; excitatory synapse; intracellular organelle; terminal button; secretory granule; presynaptic membrane; SNARE complex; synaptic vesicle; cytoplasm; plasma membrane; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; protein binding; SNARE binding; phosphatidylinositol-4,5-bisphosphate binding; syntaxin binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phospholipid binding

Biological Process: positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; regulation of synaptic transmission, glutamatergic; vesicle docking; neurotransmitter secretion; positive regulation of vesicle fusion; detection of calcium ion; response to calcium ion; positive regulation of synaptic transmission; regulation of calcium ion-dependent exocytosis; calcium ion-dependent exocytosis