SYP sirna

SYP siRNA (Rat)

Cat. Num. AAA8207264

• Gene Names: Syp; Syp1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYP; SYP siRNA (Rat); Synaptophysin; Major synaptic vesicle protein p38; SYP sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SYP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 307

• Applicable Applications for SYP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SYP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYP sirna

siRNA to inhibit SYP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981622
• NCBI GeneID: 24804
• NCBI Accession #: NP_036796.1
• NCBI GenBank Nucleotide #: NM_012664.2
• UniProt Accession #: P07825; Q499R3
• Molecular Weight: 33,311 Da
• NCBI Official Full Name: synaptophysin
• NCBI Official Synonym Full Names: synaptophysin
• NCBI Official Symbol: Syp
• NCBI Official Synonym Symbols: Syp1
• NCBI Protein Information: synaptophysin
• UniProt Protein Name: Synaptophysin
• Protein Family: Syphaxin
• UniProt Gene Name: Syp
• UniProt Entry Name: SYPH_RAT

NCBI Description

may play a role in synaptic vesicle organization and synaptic plasticity [RGD, Feb 2006]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle

Cellular Component: neuron projection; protein complex; synaptic vesicle membrane; intracellular organelle; excitatory synapse; terminal button; integral to synaptic vesicle membrane; presynaptic membrane; synaptic vesicle; membrane; presynaptic active zone; synapse; cell junction

Molecular Function: protein domain specific binding; identical protein binding; protein binding; transporter activity; syntaxin-1 binding; protein complex binding; cholesterol binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity