SUV420H1 sirna

SUV420H1 siRNA (Rat)

Cat. Num. AAA8219642

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SUV420H1; SUV420H1 siRNA (Rat); Histone-lysine N-methyltransferase SUV420H1; Suppressor of variegation 4-20 homolog 1; Su(var)4-20 homolog 1; Suv4-20h1; SUV420H1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SUV420H1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 883

• Applicable Applications for SUV420H1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SUV420H1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SUV420H1 sirna

siRNA to inhibit SUV420H1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157817845
• NCBI GeneID: 361688
• NCBI Accession #: NP_001101982.1
• NCBI GenBank Nucleotide #: NM_001108512.1
• UniProt Accession #: P0C2N5
• Molecular Weight: 98,668 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV420H1
• NCBI Official Synonym Full Names: suppressor of variegation 4-20 homolog 1 (Drosophila)
• NCBI Official Symbol: Suv420h1
• NCBI Protein Information: histone-lysine N-methyltransferase SUV420H1
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV420H1
• UniProt Gene Name: Suv420h1
• UniProt Synonym Gene Names: Su(var)4-20 homolog 1; Suv4-20h1
• UniProt Entry Name: SV421_RAT

Uniprot Description

SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase; Amino Acid Metabolism - lysine degradation

Cellular Component: condensed nuclear chromosome, pericentric region

Molecular Function: histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity

Biological Process: histone methylation; muscle development; transcription, DNA-dependent; regulation of transcription, DNA-dependent