Rabbit IPO-38 ELISA Kit | IPO-38 elisa kit
Rabbit IPO-38 ELISA Kit
Reactivity
Rabbit
Synonyms
IPO-38; Rabbit IPO-38 ELISA Kit; IPO-38 elisa kit
Reactivity
Rabbit
Assay Type
Sandwich
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IPO-38 elisa kit
Intended Uses: This IPO-38 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IPO-38in the sample, this IPO-38ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IPO-38concentration. The concentration of IPO-38in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This IPO-38enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IPO-38. Standards or samples are then added to the microtiter plate wells and IPO-38if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IPO-38present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IPO-38are added to each well to "sandwich" the IPO-38immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain IPO-38and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Principle of the Assay: This IPO-38enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IPO-38. Standards or samples are then added to the microtiter plate wells and IPO-38if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IPO-38present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IPO-38are added to each well to "sandwich" the IPO-38immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain IPO-38and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
