SETMAR sirna

SETMAR siRNA (Rat)

Cat. Num. AAA8201408

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SETMAR; SETMAR siRNA (Rat); Histone-lysine N-methyltransferase SETMAR; SET domain and mariner transposase fusion gene-containing protein homolog; SETMAR sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SETMAR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 315

• Applicable Applications for SETMAR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SETMAR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SETMAR sirna

siRNA to inhibit SETMAR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68163525
• NCBI GeneID: 500281
• NCBI Accession #: NP_001020219.1
• NCBI GenBank Nucleotide #: NM_001025048.1
• UniProt Accession #: Q5I0M0
• Molecular Weight: 35,030 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETMAR
• NCBI Official Synonym Full Names: SET domain and mariner transposase fusion gene
• NCBI Official Symbol: Setmar
• NCBI Protein Information: histone-lysine N-methyltransferase SETMAR
• UniProt Protein Name: Histone-lysine N-methyltransferase SETMAR
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Setmar
• UniProt Entry Name: SETMR_RAT

NCBI Description

This gene encodes a histone-lysine N-methyltransferase that may be involved in the methylation of histone H3. In anthropoid primates this gene is a fusion gene of a SET histone-lysine N-methyltransferase and a mariner (MAR) family transposase. In all other species this gene contains only the SET domain. [provided by RefSeq, Feb 2013]

Uniprot Description

SETMAR: Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43

Cellular Component: condensed chromosome; chromosome; nucleus

Molecular Function: structure-specific DNA binding; protein homodimerization activity; single-stranded DNA specific endodeoxyribonuclease activity; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); endonuclease activity; double-stranded DNA binding; histone-lysine N-methyltransferase activity; single-stranded DNA binding; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: cell proliferation; DNA integration; histone H3-K4 methylation; DNA double-strand break processing; histone H3-K36 methylation; DNA catabolic process, endonucleolytic; double-strand break repair via nonhomologous end joining; replication fork processing