PRDM9 sirna

PRDM9 siRNA (Human)

Cat. Num. AAA8224531

• Gene Names: PRDM9; PFM6; MSBP3; ZNF899; MEISETZ
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM9; PRDM9 siRNA (Human); PFM6; Histone-lysine N-methyltransferase PRDM9; PR domain zinc finger protein 9; PR domain-containing protein 9; PRDM9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDM9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 894

• Applicable Applications for PRDM9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM9 sirna

siRNA to inhibit PRDM9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 147905620
• NCBI GeneID: 56979
• NCBI Accession #: NP_064612.2
• NCBI GenBank Nucleotide #: NM_020227.3
• UniProt Accession #: Q9NQV7; Q27Q50; B4DX22
• Molecular Weight: 103,376 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
• NCBI Official Synonym Full Names: PR domain containing 9
• NCBI Official Symbol: PRDM9
• NCBI Official Synonym Symbols: PFM6; MSBP3; ZNF899; MEISETZ
• NCBI Protein Information: histone-lysine N-methyltransferase PRDM9
• UniProt Protein Name: Histone-lysine N-methyltransferase PRDM9
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM9
• UniProt Synonym Gene Names: PFM6
• UniProt Entry Name: PRDM9_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]

Uniprot Description

PRDM9: Histone methyltransferase that specifically trimethylates 'Lys-4' of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does not have the ability to mono- and dimethylate 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase.

Protein type: EC 2.1.1.43; C2H2-type zinc finger protein; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 5p14

Cellular Component: nucleoplasm; chromosome

Molecular Function: sequence-specific DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; meiotic gene conversion; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter