POLA1 sirna

POLA1 siRNA (Rat)

Cat. Num. AAA8226397

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLA1; POLA1 siRNA (Rat); POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; Fragment; POLA1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: POLA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,451

• Applicable Applications for POLA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat POLA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLA1 sirna

siRNA to inhibit POLA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 281604221
• NCBI GeneID: 85241
• NCBI Accession #: NP_445931.1
• NCBI GenBank Nucleotide #: NM_053479.1
• UniProt Accession #: O89042
• Molecular Weight: 165,306 Da
• NCBI Official Full Name: DNA polymerase alpha catalytic subunit
• NCBI Official Synonym Full Names: polymerase (DNA directed), alpha 1, catalytic subunit
• NCBI Official Symbol: Pola1
• NCBI Protein Information: DNA polymerase alpha catalytic subunit
• UniProt Protein Name: DNA polymerase alpha catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: Pola1
• UniProt Synonym Gene Names: Pola
• UniProt Entry Name: DPOLA_RAT

NCBI Description

exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006]

Uniprot Description

POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA repair, damage; DNA replication; Transferase; Nuclear envelope; Nucleotide Metabolism - purine

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; nuclear matrix; cytoplasm; nucleolus; nuclear envelope; nucleus; chromatin

Molecular Function: DNA primase activity; metal ion binding; DNA-directed DNA polymerase activity; nucleotide binding; protein kinase binding; DNA binding; protein heterodimerization activity; purine nucleotide binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; nucleoside binding; chromatin binding; pyrimidine nucleotide binding

Biological Process: DNA synthesis during DNA repair; cell proliferation; DNA replication initiation; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; DNA repair; leading strand elongation; DNA replication; lagging strand elongation; double-strand break repair via nonhomologous end joining