PDE2A sirna

PDE2A siRNA (Rat)

Cat. Num. AAA828202

• Gene Names: Pde2a; Pde2; Pde2a2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE2A; PDE2A siRNA (Rat); cGMP-dependent 3'.5'-cyclic phosphodiesterase; Cyclic GMP-stimulated phosphodiesterase; CGS-PDE; cGSPDE; PDE2A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PDE2A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 935

• Applicable Applications for PDE2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDE2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE2A sirna

siRNA to inhibit PDE2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 219803038
• NCBI GeneID: 81743
• NCBI Accession #: NP_001137319.1
• NCBI GenBank Nucleotide #: NM_001143847.1
• UniProt Accession #: Q01062
• Molecular Weight: – Da
• NCBI Official Full Name: cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A3
• NCBI Official Synonym Full Names: phosphodiesterase 2A, cGMP-stimulated
• NCBI Official Symbol: Pde2a
• NCBI Official Synonym Symbols: Pde2; Pde2a2
• NCBI Protein Information: cGMP-dependent 3',5'-cyclic phosphodiesterase
• UniProt Protein Name: cGMP-dependent 3',5'-cyclic phosphodiesterase
• Protein Family: cGMP-dependent 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde2a
• UniProt Synonym Gene Names: CGS-PDE; cGSPDE
• UniProt Entry Name: PDE2A_RAT

NCBI Description

activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006]

Uniprot Description

PDE2A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Mitochondrial

Cellular Component: Golgi apparatus; endoplasmic reticulum; dendrite; cytosol; lipid raft; presynaptic membrane; mitochondrial matrix; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; intracellular; nucleus

Molecular Function: protein binding; protein homodimerization activity; TPR domain binding; metal ion binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity; drug binding; cAMP binding

Biological Process: monocyte differentiation; metabolic process; cGMP-mediated signaling; cAMP catabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vascular permeability; cAMP-mediated signaling; negative regulation of cAMP biosynthetic process; negative regulation of protein import into nucleus, translocation; negative regulation of vascular permeability; cGMP catabolic process; regulation of cGMP metabolic process; protein targeting to mitochondrion; positive regulation of inflammatory response