PARP1 sirna

PARP1 siRNA (Rat)

Cat. Num. AAA8238354

• Gene Names: Parp1; ARTD1; Adprt; Parp-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PARP1; PARP1 siRNA (Rat); ADPRT; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PARP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1014

• Applicable Applications for PARP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PARP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PARP1 sirna

siRNA to inhibit PARP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978455
• NCBI GeneID: 25591
• NCBI Accession #: NP_037195.1
• NCBI GenBank Nucleotide #: NM_013063.2
• UniProt Accession #: P27008; O35937
• Molecular Weight: 112,660 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase 1
• NCBI Official Symbol: Parp1
• NCBI Official Synonym Symbols: ARTD1; Adprt; Parp-1
• NCBI Protein Information: poly [ADP-ribose] polymerase 1
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: Parp1
• UniProt Synonym Gene Names: Adprt; PARP-1; ARTD1; ADPRT 1
• UniProt Entry Name: PARP1_RAT

NCBI Description

catalyzes poly (ADP-ribose) protein modification; plays a role in DNA repair and genome stability [RGD, Feb 2006]

Uniprot Description

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Protein type: DNA repair, damage; Nucleolus; Transferase; EC 2.4.2.30; Nuclear envelope; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; transcription factor complex; protein complex; membrane; cytoplasm; nucleolus; nuclear envelope; nucleus

Molecular Function: identical protein binding; enzyme binding; DNA binding; zinc ion binding; transferase activity, transferring pentosyl groups; protein N-terminus binding; protein kinase binding; NAD binding; transcription factor binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein autoprocessing; protein amino acid ADP-ribosylation; transcription, DNA-dependent; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; DNA damage response, detection of DNA damage; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; DNA metabolic process; telomere maintenance