Typical Testing Data/Standard Curve (for reference only)
Rat Poly [ADP-ribose] polymerase 1 ELISA Kit | Parp1 elisa kit
Rat Poly [ADP-ribose] polymerase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PARP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PARP-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PARP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PARP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
catalyzes poly (ADP-ribose) protein modification; plays a role in DNA repair and genome stability [RGD, Feb 2006]
Uniprot Description
PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
Protein type: Transferase; Nuclear envelope; EC 2.4.2.30; DNA repair, damage; Nuclear receptor co-regulator; Nucleolus
Cellular Component: cytoplasm; membrane; mitochondrion; nuclear chromosome, telomeric region; nuclear envelope; nucleolus; nucleoplasm; nucleus; protein complex; transcription factor complex
Molecular Function: chromatin binding; DNA binding; DNA ligase (ATP) activity; enzyme binding; estrogen receptor binding; histone deacetylase binding; identical protein binding; NAD binding; NAD+ ADP-ribosyltransferase activity; protein binding; protein kinase binding; protein N-terminus binding; SMAD binding; transcription factor binding; transferase activity, transferring pentosyl groups; zinc ion binding
Biological Process: base-excision repair; behavioral response to cocaine; cellular response to insulin stimulus; DNA damage response, detection of DNA damage; DNA ligation during DNA repair; DNA metabolic process; double-strand break repair; lagging strand elongation; mitochondrial DNA metabolic process; mitochondrial DNA repair; mitochondrion organization and biogenesis; positive regulation of estrogen receptor signaling pathway; positive regulation of mitochondrial depolarization; positive regulation of transcription from RNA polymerase II promoter; protein amino acid ADP-ribosylation; protein autoprocessing; regulation of growth rate; response to DNA damage stimulus; response to gamma radiation; telomere maintenance; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway
Research Articles on Parp1
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Product Notes
The Rat Parp1 parp1 (Catalog #AAA2884603) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884603 ELISA Kit recognizes Rat Parp1. It is sometimes possible for the material contained within the vial of "Poly [ADP-ribose] polymerase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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