NEDD4 sirna

NEDD4 siRNA (Rat)

Cat. Num. AAA8202943

• Gene Names: Nedd4; Nedd4a
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NEDD4; NEDD4 siRNA (Rat); NEDD4A; E3 ubiquitin-protein ligase NEDD4; NEDD4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NEDD4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 889

• Applicable Applications for NEDD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NEDD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NEDD4 sirna

siRNA to inhibit NEDD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158186672
• NCBI GeneID: 25489
• NCBI Accession #: NP_037118.1
• NCBI GenBank Nucleotide #: NM_012986.1
• UniProt Accession #: Q62940
• Molecular Weight: 102,395 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase NEDD4
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• NCBI Official Symbol: Nedd4
• NCBI Official Synonym Symbols: Nedd4a
• NCBI Protein Information: E3 ubiquitin-protein ligase NEDD4
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: Nedd4
• UniProt Synonym Gene Names: Nedd4a
• UniProt Entry Name: NEDD4_RAT

NCBI Description

may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]

Uniprot Description

NEDD4: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR- 2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, TMEPAI and PRRG2. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes. Interaction with PTEN is questionable according to PubMed:18562292. Interacts with viral proteins that contain a late- budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag. Interacts (via C2 domain) with GRB10 (via SH2 domain). Interacts with ERBB4. Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Activated by NDFIP1- and NDFIP2-binding. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Nuclear receptor co-regulator; Ubiquitin ligase; Ligase

Cellular Component: Golgi apparatus; microvillus; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cell cortex; nucleus; cytosol; chromatin; ubiquitin ligase complex; lipid raft

Molecular Function: protein C-terminus binding; sodium channel inhibitor activity; protein domain specific binding; protein binding; ubiquitin binding; phosphothreonine binding; beta-2 adrenergic receptor binding; ubiquitin-protein ligase activity; phosphoserine binding; ligase activity

Biological Process: protein monoubiquitination; protein targeting to lysosome; lysosomal transport; adaptive immune response; progesterone receptor signaling pathway; negative regulation of vascular endothelial growth factor receptor signaling pathway; T cell activation; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; transmission of virus; regulation of dendrite morphogenesis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; positive regulation of nucleocytoplasmic transport; positive regulation of phosphoinositide 3-kinase cascade; regulation of membrane potential; receptor catabolic process; receptor internalization; glucocorticoid receptor signaling pathway; positive regulation of protein catabolic process; regulation of synapse organization and biogenesis; blood vessel morphogenesis; immune response; neurite development; neuromuscular junction development