MIF sirna

MIF siRNA (Rat)

Cat. Num. AAA8215805

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MIF; MIF siRNA (Rat); Macrophage migration inhibitory factor; Glutathione-binding 13 kDa protein; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase; MIF sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MIF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 115

• Applicable Applications for MIF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MIF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MIF sirna

siRNA to inhibit MIF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13591985
• NCBI GeneID: 81683
• NCBI Accession #: NP_112313.1
• NCBI GenBank Nucleotide #: NM_031051.1
• UniProt Accession #: P30904
• Molecular Weight: 12,477 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: Mif
• NCBI Protein Information: macrophage migration inhibitory factor
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: MIF-like protein
• UniProt Gene Name: Mif
• UniProt Synonym Gene Names: MIF
• UniProt Entry Name: MIF_RAT

NCBI Description

inhibits random migration of macrophages and is involved in the pathogenesis of several inflammatory diseases [RGD, Feb 2006]

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - phenylalanine; Cytokine; EC 5.3.3.12; EC 5.3.2.1; Cell development/differentiation; Isomerase; Apoptosis; Amino Acid Metabolism - tyrosine

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus; vesicle; myelin sheath

Molecular Function: phenylpyruvate tautomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein complex binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding

Biological Process: skin development; metabolic process; response to menaquinone; positive regulation of potassium ion transport; response to glucocorticoid stimulus; response to lipopolysaccharide; prostaglandin biosynthetic process; positive regulation of glucose import; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; negative regulation of myeloid cell apoptosis; positive regulation of fibroblast proliferation; response to vitamin E; cell surface receptor linked signal transduction; positive chemotaxis; hair follicle development; positive regulation of acute inflammatory response; positive regulation of B cell proliferation; response to drug; positive regulation of axon regeneration; cell aging; brain renin-angiotensin system; positive regulation of peptidyl-tyrosine phosphorylation; response to mechanical stimulus; response to heat; response to progesterone stimulus; negative regulation of apoptosis; positive regulation of immune response; wound healing; DNA damage response, signal transduction by p53 class mediator; drinking behavior; response to hormone stimulus; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of cellular protein metabolic process; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; negative regulation of mature B cell apoptosis; inflammatory response; aging; response to inorganic substance; carboxylic acid metabolic process; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; cell proliferation; positive regulation of glycolysis; innate immune response; spermatogenesis; positive regulation of protein amino acid phosphorylation; brain development; positive regulation of phosphorylation; positive regulation of cytokine secretion