Rabbit anti-Human MYL2 Polyclonal Antibody | anti-MYL2 antibody

MYL2 Antibody

Cat. Num. AAA7000579

• Gene Names: MYL2; MLC2; CMH10; MLC-2s/v
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%,Protein G purified
• Synonyms: MYL2; Polyclonal Antibody; MYL2 Antibody; Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; Cardiac myosin light chain 21; anti-MYL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 166

• Applicable Applications for anti-MYL2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-34490
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS7000579 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG2 cells using MBS7000579 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-MYL2 antibody

Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI Accession #: NP_000423.2
• NCBI GenBank Nucleotide #: NM_000432.3
• UniProt Accession #: P10916; Q16123
• Molecular Weight: 18,789 Da
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin light chain 2
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10; MLC-2s/v
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin regulatory
• UniProt Gene Name: MYL2
• UniProt Synonym Gene Names: MLC-2s/v
• UniProt Entry Name: MLRV_HUMAN

NCBI Description

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: A band; actin cytoskeleton; cytoskeleton; cytosol; myofibril; myosin complex; sarcomere

Molecular Function: actin monomer binding; calcium ion binding; myosin heavy chain binding; protein binding; structural constituent of muscle

Biological Process: cardiac muscle contraction; cardiac myofibril assembly; heart contraction; heart development; muscle cell fate specification; muscle fiber development; muscle filament sliding; negative regulation of cell growth; post-embryonic development; regulation of striated muscle contraction; regulation of the force of heart contraction; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Familial Hypertrophic, 10