Macrophage Migration Inhibitory Factor (MIF) Recombinant Protein | MIF recombinant protein

Recombinant Macrophage Migration Inhibitory Factor (MIF)

Cat. Num. AAA2012476

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Macrophage Migration Inhibitory Factor (MIF); Recombinant Macrophage Migration Inhibitory Factor (MIF); MIF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-FIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GS
• Sequence Length: 115

• Applicable Applications for MIF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Phe4~Ser112 (Accession # P30904) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13591985
• NCBI GeneID: 81683
• NCBI Accession #: NP_112313.1
• NCBI GenBank Nucleotide #: NM_031051.1
• UniProt Accession #: P30904
• Molecular Weight: 13.3kDa
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: Mif
• NCBI Protein Information: macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glutathione-binding 13 kDa protein
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: Macrophage migration inhibitory factor
• UniProt Gene Name: Mif
• UniProt Synonym Gene Names: MIF
• UniProt Entry Name: MIF_RAT

NCBI Description

inhibits random migration of macrophages and is involved in the pathogenesis of several inflammatory diseases [RGD, Feb 2006]

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: EC 5.3.3.12; EC 5.3.2.1; Amino Acid Metabolism - phenylalanine; Isomerase; Apoptosis; Cell development/differentiation; Cytokine; Amino Acid Metabolism - tyrosine

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus; vesicle; myelin sheath

Molecular Function: phenylpyruvate tautomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; protein complex binding; dopachrome isomerase activity; receptor binding; chemoattractant activity

Biological Process: skin development; metabolic process; response to menaquinone; response to glucocorticoid stimulus; positive regulation of potassium ion transport; response to lipopolysaccharide; prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; positive regulation of glucose import; response to vitamin E; positive regulation of fibroblast proliferation; negative regulation of myeloid cell apoptosis; cell surface receptor linked signal transduction; positive chemotaxis; hair follicle development; positive regulation of acute inflammatory response; positive regulation of B cell proliferation; response to drug; positive regulation of axon regeneration; cell aging; brain renin-angiotensin system; positive regulation of peptidyl-tyrosine phosphorylation; response to mechanical stimulus; response to heat; response to progesterone stimulus; negative regulation of apoptosis; positive regulation of immune response; DNA damage response, signal transduction by p53 class mediator; wound healing; drinking behavior; positive regulation of lipopolysaccharide-mediated signaling pathway; response to hormone stimulus; negative regulation of cellular protein metabolic process; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; negative regulation of mature B cell apoptosis; inflammatory response; aging; response to inorganic substance; carboxylic acid metabolic process; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; cell proliferation; positive regulation of glycolysis; innate immune response; spermatogenesis; positive regulation of protein amino acid phosphorylation; brain development; positive regulation of phosphorylation; positive regulation of cytokine secretion