LMAN1 sirna

LMAN1 siRNA (Rat)

Cat. Num. AAA8236117

• Gene Names: Lman1; p58
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LMAN1; LMAN1 siRNA (Rat); ERGIC53; Protein ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; Lectin mannose-binding 1; p58; LMAN1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: LMAN1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 517

• Applicable Applications for LMAN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LMAN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LMAN1 sirna

siRNA to inhibit LMAN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758758
• NCBI GeneID: 116666
• NCBI Accession #: NP_446338.1
• NCBI GenBank Nucleotide #: NM_053886.2
• UniProt Accession #: Q62902
• Molecular Weight: 57,957 Da
• NCBI Official Full Name: protein ERGIC-53
• NCBI Official Synonym Full Names: lectin, mannose-binding, 1
• NCBI Official Symbol: Lman1
• NCBI Official Synonym Symbols: p58
• NCBI Protein Information: protein ERGIC-53
• UniProt Protein Name: Protein ERGIC-53
• Protein Family: Protein
• UniProt Gene Name: Lman1
• UniProt Synonym Gene Names: Ergic53
• UniProt Entry Name: LMAN1_RAT

NCBI Description

calcium-dependent lectin; involved in glycoprotein export from the ER [RGD, Feb 2006]

Uniprot Description

LMAN1: Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1); also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.

Protein type: Membrane protein, integral

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; sarcomere; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; ER-Golgi intermediate compartment; ER to Golgi transport vesicle

Molecular Function: mannose binding; identical protein binding; calcium ion binding

Biological Process: endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; protein transport; protein exit from endoplasmic reticulum; Golgi organization and biogenesis