KYNU sirna

KYNU siRNA (Rat)

Cat. Num. AAA8210758

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KYNU; KYNU siRNA (Rat); Kynureninase; L-kynurenine hydrolase; KYNU sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KYNU siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 464

• Applicable Applications for KYNU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KYNU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KYNU sirna

siRNA to inhibit KYNU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758776
• NCBI GeneID: 116682
• NCBI Accession #: NP_446354.1
• NCBI GenBank Nucleotide #: NM_053902.2
• UniProt Accession #: P70712; Q68G25; Q7M0D0; Q9QW90
• Molecular Weight: 52,470 Da
• NCBI Official Full Name: kynureninase
• NCBI Official Synonym Full Names: kynureninase
• NCBI Official Symbol: Kynu
• NCBI Protein Information: kynureninase
• UniProt Protein Name: Kynureninase
• Protein Family: Kynureninase
• UniProt Gene Name: Kynu
• UniProt Entry Name: KYNU_RAT

NCBI Description

enzyme involved in the biosynthesis of NAD cofactors from tryptophan [RGD, Feb 2006]

Uniprot Description

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Hydrolase; Amino Acid Metabolism - tryptophan; EC 3.7.1.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; kynureninase activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; quinolinate biosynthetic process; tryptophan catabolic process