Rat Kynureninase ELISA Kit | Kynu elisa kit

Rat Kynureninase ELISA Kit

Cat. Num. AAA2880764

• Reactivity: Rat
• Synonyms: Kynureninase; Rat Kynureninase ELISA Kit; L-kynurenine hydrolase; Kynu; 3.7.1.3; Kynu elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Kynureninase
• Sequence Length: 464

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: <0.159 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.1%
• Inter-assay Precision: Inter-Assay CV: <=7.8%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Kynu elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16758776
• NCBI GeneID: 116682
• NCBI Accession #: NP_446354.1
• NCBI GenBank Nucleotide #: NM_053902.2
• UniProt Accession #: P70712; Q68G25; Q7M0D0; Q9QW90
• Molecular Weight: 52,470 Da
• NCBI Official Full Name: kynureninase
• NCBI Official Synonym Full Names: kynureninase
• NCBI Official Symbol: Kynu
• NCBI Protein Information: kynureninase
• UniProt Protein Name: Kynureninase
• Protein Family: Kynureninase
• UniProt Gene Name: Kynu
• UniProt Entry Name: KYNU_RAT

NCBI Description

enzyme involved in the biosynthesis of NAD cofactors from tryptophan [RGD, Feb 2006]

Uniprot Description

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; EC 3.7.1.3; Amino Acid Metabolism - tryptophan

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm

Molecular Function: kynureninase activity; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; quinolinate biosynthetic process; tryptophan catabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine