INPP5D sirna

INPP5D siRNA (Rat)

Cat. Num. AAA8229896

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INPP5D; INPP5D siRNA (Rat); SHIP; SHIP1; Phosphatidylinositol 3.4.5-trisphosphate 5-phosphatase 1; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; INPP5D sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: INPP5D siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1190

• Applicable Applications for INPP5D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat INPP5D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INPP5D sirna

siRNA to inhibit INPP5D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9506813
• NCBI GeneID: 54259
• NCBI Accession #: NP_062184.1
• NCBI GenBank Nucleotide #: NM_019311.1
• UniProt Accession #: P97573
• Molecular Weight: 133,593 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase D
• NCBI Official Symbol: Inpp5d
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
• UniProt Gene Name: Inpp5d
• UniProt Synonym Gene Names: Ship; Ship1; SH2 domain-containing inositol phosphatase 1; SHIP-1
• UniProt Entry Name: SHIP1_RAT

NCBI Description

phosphatase involved in the inhibitory action of the mast cell function-associated antigen [RGD, Feb 2006]

Uniprot Description

SHIP: an SH2-containing inositol phosphatase. A hemopoietic-specific phosphatase that regulates cell survival, growth, cell cycle arrest and apoptosis. Hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. A cytosolic protein with a SH2 domain in its N-terminus and two NPXY Shc binding motifs at its C-terminus. Upon receptor cross-linking, SHIP is first recruited to the plasma membrane by its SH2, followed by tyrosine phosphorylation on the NPXY motif. Both membrane relocalization and phosphorylation are essential for its regulatory function. Associates with SHC after cytokine stimulation.

Protein type: Phosphatase, lipid; EC 3.1.3.86; Motility/polarity/chemotaxis

Cellular Component: cortical cytoskeleton; plasma membrane; actin filament; intracellular; cytosol

Molecular Function: protein binding; PTB domain binding; inositol-4,5-bisphosphate 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity; SH3 domain binding

Biological Process: negative regulation of osteoclast differentiation; apoptosis; positive regulation of apoptosis; immune system process; positive regulation of erythrocyte differentiation; negative regulation of immune response; negative regulation of B cell activation; negative regulation of interleukin-6 biosynthetic process; determination of adult life span; negative regulation of B cell proliferation; negative regulation of monocyte differentiation; negative regulation of neutrophil differentiation; negative regulation of granulocyte differentiation; negative regulation of signal transduction; negative regulation of cell proliferation; positive regulation of lymphocyte differentiation; dephosphorylation; phosphoinositide dephosphorylation; positive regulation of B cell differentiation; immunoglobulin mediated immune response; negative regulation of bone resorption