Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kappaB p65 antibody and NFkB p65 antibody (Ser529) (Lanes 3 and 4))
Rabbit anti-Mouse NFkB p65 Polyclonal Antibody | anti-NFkB antibody
NFkB p65 Antibody (Ser529)
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
NFkB p65; Polyclonal Antibody; NFkB p65 Antibody (Ser529); Rabbit Polyclonal NFkB p65 Antibody (Ser529); Polyclonal NFkB p65 antibody; Anti-NFkB p65 antibody; MGC131774 antibody; NFKB3 antibody; Nuclear factor NF-kappa-B p65 subunit antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody; p65 antibody; RELA antibody; TF65 antibody; Transcription factor p65 antibody; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)) antibody; v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody; v-rel reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65 antibody; anti-NFkB antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NFkB p65 antibody (Ser529) detects endogenous levels of NFkB p65 protein only when phosphorylated at serine 529. The antibody will detect species specific phosphorylation as follows: Human: Ser529, Mouse: Ser529, Rat: Ser529
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-NFkB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
NFkB p65 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human NFkB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kappaB p65 antibody and NFkB p65 antibody (Ser529) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kappaB p65 antibody and NFkB p65 antibody (Ser529) (Lanes 3 and 4))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB p65 antibody (Ser529))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB p65 antibody (Ser529))
Related Product Information for anti-NFkB antibody
NF-kB functions as a fast messenger. Under normal circumstances, it is sequestered in the cytoplasm by an inhibitor protein. Additionally, the p50 subunit is in the inactive elongated p105 state. Once a viral infection is recognized, the inhibitor is phosphorylated, releasing the complex, and the repeated chain is cleaved. Because the proteins originate in the cytoplasm already assembled and proceed to the nucleus where they act as the transcription factor, the cellular message is very fast.
Product Categories/Family for anti-NFkB antibody
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Product Notes
The NFkB (Catalog #AAA5313643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFkB p65 Antibody (Ser529) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the NFkB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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