GNB2L1 sirna

GNB2L1 siRNA (Rat)

Cat. Num. AAA8204314

• Gene Names: Gnb2l1; RACK1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GNB2L1; GNB2L1 siRNA (Rat); Guanine nucleotide-binding protein subunit beta-2-like 1; Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1; GNB2L1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GNB2L1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for GNB2L1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GNB2L1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GNB2L1 sirna

siRNA to inhibit GNB2L1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 406035317
• NCBI GeneID: 83427
• NCBI Accession #: NP_570090.2
• NCBI GenBank Nucleotide #: NM_130734.2
• UniProt Accession #: P63245; P25388; P99049
• Molecular Weight: 35,077 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-2-like 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1
• NCBI Official Symbol: Gnb2l1
• NCBI Official Synonym Symbols: RACK1
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Gene Name: Gnb2l1
• UniProt Synonym Gene Names: RACK1
• UniProt Entry Name: GBLP_RAT

NCBI Description

may act as a receptor for activated protein kinase C [RGD, Feb 2006]

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric beta; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Adaptor/scaffold; G protein

Cellular Component: small ribosomal subunit; mitochondrion; dendrite; perikaryon; cytosol; cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; midbody; nucleus; phagocytic cup

Molecular Function: protein tyrosine kinase inhibitor activity; enzyme binding; protein homodimerization activity; protein kinase C binding; caspase activator activity; SH2 domain binding; receptor tyrosine kinase binding; protein phosphatase binding; ion channel inhibitor activity

Biological Process: caspase activation; negative regulation of phagocytosis; negative regulation of Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; positive regulation of protein homooligomerization; positive regulation of apoptosis; regulation of cell cycle; rhythmic process; gastrulation; osteoblast differentiation; regulation of protein localization; positive regulation of mitochondrial depolarization; negative regulation of translation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein kinase C activation; positive regulation of cAMP catabolic process; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; regulation of cell division; positive regulation of cell migration; positive regulation of GTPase activity