Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat FOXI1/FKHL10 Polyclonal Antibody | anti-FOXI1 antibody
Goat anti-FOXI1/FKHL10 Antibody
Immunohistochemistry: In paraffin embedded Human Testis shows primarily nuclear staining in the primary spermatocytes. Recommended concentration, 2-4ug/ml. This product has been successfully used in Mouse Epididymus by anonymous customer.
Western Blot: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41. 0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXI1: Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: nucleus
Molecular Function: DNA bending activity; sequence-specific DNA binding; transcription factor activity
Biological Process: embryonic development; inner ear morphogenesis; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Disease: Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct; Pendred Syndrome
Research Articles on FOXI1
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Product Notes
The FOXI1 foxi1 (Catalog #AAA420822) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXI1/FKHL10 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXI1/FKHL10 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Testis shows primarily nuclear staining in the primary spermatocytes. Recommended concentration, 2-4ug/ml. This product has been successfully used in Mouse Epididymus by anonymous customer. Western Blot: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41. 0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the FOXI1 foxi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TSGVLYPREG TEV. It is sometimes possible for the material contained within the vial of "FOXI1/FKHL10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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