G6PDX sirna

G6PDX siRNA (Rat)

Cat. Num. AAA8226307

• Gene Names: G6pd; G6pdx
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G6PDX; G6PDX siRNA (Rat); G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PDX sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: G6PDX siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 515

• Applicable Applications for G6PDX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat G6PDX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G6PDX sirna

siRNA to inhibit G6PDX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393381
• NCBI GeneID: 24377
• NCBI Accession #: NP_058702.1
• NCBI GenBank Nucleotide #: NM_017006.2
• UniProt Accession #: P05370
• Molecular Weight: 59,376 Da
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6pd
• NCBI Official Synonym Symbols: G6pdx
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• Protein Family: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6pdx
• UniProt Synonym Gene Names: G6pd; G6PD
• UniProt Entry Name: G6PD_RAT

NCBI Description

catalyzes the first rate-limiting step in the pentose phosphate pathway; may mediate peroxynitrite functions in neuroprotection against nitric oxide-mediated apoptosis [RGD, Feb 2006]

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: EC 1.1.1.49; Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - pentose phosphate pathway; Cell development/differentiation; Oxidoreductase

Cellular Component: centrosome; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus; cytosol

Molecular Function: identical protein binding; glucose-6-phosphate dehydrogenase activity; protein homodimerization activity; carbohydrate binding; NADP binding; glucose binding

Biological Process: response to food; NADPH regeneration; pentose-phosphate shunt; erythrocyte development; ribose phosphate biosynthetic process; erythrocyte maturation; interleukin-12 production; glucose 6-phosphate metabolic process; pentose-phosphate shunt, oxidative branch; regulation of multicellular organism growth; glucose metabolic process; cytokine production; NADP biosynthetic process; cholesterol biosynthetic process; response to organic cyclic substance; NADP metabolic process; angiotensin mediated vasodilation involved in regulation of systemic arterial blood pressure; glutathione metabolic process; substantia nigra development; response to ethanol; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; regulation of neuron apoptosis; interleukin-10 production; response to oxidative stress; pentose biosynthetic process