FXYD1 sirna

FXYD1 siRNA (Rat)

Cat. Num. AAA8207383

• Gene Names: Fxyd1; Plm
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FXYD1; FXYD1 siRNA (Rat); PLM; Phospholemman; FXYD domain-containing ion transport regulator 1; FXYD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: FXYD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 92

• Applicable Applications for FXYD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FXYD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FXYD1 sirna

siRNA to inhibit FXYD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 564327531
• NCBI GeneID: 58971
• NCBI Accession #: XP_006228901.1
• NCBI GenBank Nucleotide #: XM_006228839.2
• UniProt Accession #: O08589
• Molecular Weight: 10,365 Da
• NCBI Official Full Name: phospholemman isoform X2
• NCBI Official Synonym Full Names: FXYD domain-containing ion transport regulator 1
• NCBI Official Symbol: Fxyd1
• NCBI Official Synonym Symbols: Plm
• NCBI Protein Information: phospholemman
• UniProt Protein Name: Phospholemman
• Protein Family: Phospholemman
• UniProt Gene Name: Fxyd1
• UniProt Synonym Gene Names: Plm
• UniProt Entry Name: PLM_RAT

NCBI Description

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. [provided by RefSeq, Jul 2008]

Uniprot Description

PLM: a protein of the FXYD family of small ion transport regulators or channels. Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May have a functional role in muscle contraction. Major plasma membrane substrate for PKA and PKC in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation.Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining.

Protein type: Cell surface; Channel, misc.; Membrane protein, integral

Cellular Component: plasma membrane; caveola; sarcolemma

Molecular Function: protein binding; chloride channel activity; myosin binding; sodium channel regulator activity; ATPase binding

Biological Process: positive regulation of organic acid transport; cytosolic calcium ion homeostasis; brain development