Rabbit PHKG2 Polyclonal Antibody | anti-PHKG2 antibody

PHKG2 Polyclonal Antibody

Cat. Num. AAA9134050

• Gene Names: PHKG2; GSD9C
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: PHKG2; Polyclonal Antibody; PHKG2 Polyclonal Antibody; GSD9C; anti-PHKG2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence: RQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIMGPEEEGDSAAITEDEAVLVLG
• Sequence Length: 406

• Applicable Applications for anti-PHKG2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human PHKG2
• Immunogen Species: Human
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Positive Samples: MCF-7, 22Rv1, HepG2, Jurkat, 293T, mouse liver, mouse testis, mouse brain, rat liver
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PHKG2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Related Product Information for anti-PHKG2 antibody

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Product Categories/Family for anti-PHKG2 antibody

Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4505785
• NCBI GeneID: 5261
• NCBI Accession #: NP_000285.1
• NCBI GenBank Nucleotide #: NM_000294.2
• UniProt Accession #: P15735; P11800; A8K0C7; B4DEB7; E9PEU3
• Molecular Weight: Calculated: 43kDa; 46kDa; Observed: 46kDa
• NCBI Official Full Name: phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
• NCBI Official Synonym Full Names: phosphorylase kinase catalytic subunit gamma 2
• NCBI Official Symbol: PHKG2
• NCBI Official Synonym Symbols: GSD9C
• NCBI Protein Information: phosphorylase b kinase gamma catalytic chain, liver/testis isoform
• UniProt Protein Name: Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
• Protein Family: Phosphorylase b kinase
• UniProt Gene Name: PHKG2
• UniProt Synonym Gene Names: PHK-gamma-LT; PHK-gamma-T

NCBI Description

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM ().