EPM2A sirna

EPM2A siRNA (Rat)

Cat. Num. AAA8239517

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPM2A; EPM2A siRNA (Rat); Laforin; Glucan phosphatase; Lafora PTPase; LAFPTPase; EPM2A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: EPM2A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 331

• Applicable Applications for EPM2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat EPM2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPM2A sirna

siRNA to inhibit EPM2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 454522491
• NCBI GeneID: 114005
• NCBI Accession #: NP_001263691.1
• NCBI GenBank Nucleotide #: NM_001276762.1
• UniProt Accession #: Q91XQ2; F1LPW7
• Molecular Weight: 37,144 Da
• NCBI Official Full Name: laforin
• NCBI Official Synonym Full Names: epilepsy, progressive myoclonus type 2A
• NCBI Official Symbol: Epm2a
• NCBI Protein Information: laforin
• UniProt Protein Name: Laforin
• Protein Family: EPM2A-interacting protein
• UniProt Gene Name: Epm2a
• UniProt Synonym Gene Names: LAFPTPase
• UniProt Entry Name: EPM2A_RAT

NCBI Description

encodes a dual-specificity phosphatase (DSP) named laforin [RGD, Feb 2006]

Uniprot Description

Has both dual-specificity protein phosphatase and glucan phosphatase activities. Together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway ().