EIF2B4 sirna

EIF2B4 siRNA (Rat)

Cat. Num. AAA8226873

• Gene Names: Eif2b4; Eif2b
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF2B4; EIF2B4 siRNA (Rat); EIF2BD; Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; EIF2B4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: EIF2B4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 524

• Applicable Applications for EIF2B4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat EIF2B4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF2B4 sirna

siRNA to inhibit EIF2B4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758830
• NCBI GeneID: 117019
• NCBI Accession #: NP_446402.1
• NCBI GenBank Nucleotide #: NM_053950.1
• UniProt Accession #: Q63186
• Molecular Weight: 57,809 Da
• NCBI Official Full Name: translation initiation factor eIF-2B subunit delta
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 4 delta
• NCBI Official Symbol: Eif2b4
• NCBI Official Synonym Symbols: Eif2b
• NCBI Protein Information: translation initiation factor eIF-2B subunit delta
• UniProt Protein Name: Translation initiation factor eIF-2B subunit delta
• Protein Family: Translation initiation factor
• UniProt Gene Name: Eif2b4
• UniProt Synonym Gene Names: Eif2bd
• UniProt Entry Name: EI2BD_RAT

NCBI Description

mediates the exchange of GDP bound to translation initiation factor eIF2 for GTP [RGD, Feb 2006]

Uniprot Description

eIF2B-delta: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B4 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Translation initiation; Motility/polarity/chemotaxis

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm

Molecular Function: translation initiation factor binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity

Biological Process: myelination; regulation of translation; cellular metabolic process; response to peptide hormone stimulus; ovarian follicle development; methionine salvage; response to heat; response to glucose stimulus; translational initiation; negative regulation of translation initiation in response to stress; oligodendrocyte development; positive regulation of GTPase activity