Western Blot (WB)
(Western Blot analysis of BIRC2 expression in transfected 293T cell line by BIRC2 polyclonal antibody. Lane 1: BIRC2 transfected lysate (69.9kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human BIRC2 Polyclonal Antibody | anti-BIRC2 antibody
BIRC2 (Baculoviral IAP Repeat-containing Protein 2, C-IAP1, IAP Homolog B, Inhibitor of Apoptosis Protein 2, IAP-2, hIAP-2, hIAP2, RING Finger Protein 48, TNFR2-TRAF-signaling Complex Protein 2, API1, IAP2, MIHB, RNF48) (Biotin)
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BIRC2; Polyclonal Antibody; BIRC2 (Baculoviral IAP Repeat-containing Protein 2; C-IAP1; IAP Homolog B; Inhibitor of Apoptosis Protein 2; IAP-2; hIAP-2; hIAP2; RING Finger Protein 48; TNFR2-TRAF-signaling Complex Protein 2; API1; IAP2; MIHB; RNF48) (Biotin); anti-BIRC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BIRC2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-BIRC2 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human BIRC2, aa1-618 (NP_001157.1).
Immunogen Sequence
MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of BIRC2 expression in transfected 293T cell line by BIRC2 polyclonal antibody. Lane 1: BIRC2 transfected lysate (69.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of BIRC2 expression in transfected 293T cell line by BIRC2 polyclonal antibody. Lane 1: BIRC2 transfected lysate (69.9kD). Lane 2: Non-transfected lysate.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between BIRC2 and CASP9 HeLa cells were stained with BIRC2 rabbit purified polyclonal 1:1200 and CASP9 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between BIRC2 and CASP9 HeLa cells were stained with BIRC2 rabbit purified polyclonal 1:1200 and CASP9 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Related Product Information for anti-BIRC2 antibody
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3,-7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-a by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
Product Categories/Family for anti-BIRC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
618
NCBI Official Full Name
baculoviral IAP repeat-containing protein 2 isoform 1
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 2
NCBI Official Symbol
BIRC2
NCBI Official Synonym Symbols
API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
NCBI Protein Information
baculoviral IAP repeat-containing protein 2; IAP-2; IAP homolog B; apoptosis inhibitor 1; RING finger protein 48; inhibitor of apoptosis protein 2; baculoviral IAP repeat-containing 2; NFR2-TRAF signalling complex protein; TNFR2-TRAF-signaling complex pro
UniProt Protein Name
Baculoviral IAP repeat-containing protein 2
Protein Family
UniProt Gene Name
BIRC2
UniProt Synonym Gene Names
API1; IAP2; MIHB; RNF48; IAP-2; hIAP-2; hIAP2
UniProt Entry Name
BIRC2_HUMAN
Similar Products
Product Notes
The BIRC2 birc2 (Catalog #AAA6371257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BIRC2 (Baculoviral IAP Repeat-containing Protein 2, C-IAP1, IAP Homolog B, Inhibitor of Apoptosis Protein 2, IAP-2, hIAP-2, hIAP2, RING Finger Protein 48, TNFR2-TRAF-signaling Complex Protein 2, API1, IAP2, MIHB, RNF48) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BIRC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BIRC2 birc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIRC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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