EIF2AK3 sirna

EIF2AK3 siRNA (Rat)

Cat. Num. AAA8225491

• Gene Names: Eif2ak3; PEK
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF2AK3; EIF2AK3 siRNA (Rat); PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; EIF2AK3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: EIF2AK3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1108

• Applicable Applications for EIF2AK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat EIF2AK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF2AK3 sirna

siRNA to inhibit EIF2AK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13928816
• NCBI GeneID: 29702
• NCBI Accession #: NP_113787.1
• NCBI GenBank Nucleotide #: NM_031599.2
• UniProt Accession #: Q9Z1Z1
• Molecular Weight: 124,770 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 3
• NCBI Official Symbol: Eif2ak3
• NCBI Official Synonym Symbols: PEK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• Protein Family: Eukaryotic translation initiation factor 2-alpha kinase
• UniProt Gene Name: Eif2ak3
• UniProt Synonym Gene Names: Pek; Perk
• UniProt Entry Name: E2AK3_RAT

NCBI Description

kinase that phosphorylates eukaryotic initiation factor 2alpha; has an important role in translational control [RGD, Feb 2006]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: EC 2.7.11.1; Protein kinase, Other; Translation; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family; PEK subfamily

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cell; cytoplasm; integral to membrane; intracellular

Molecular Function: identical protein binding; protein serine/threonine kinase activity; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: fat cell differentiation; lactation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; negative regulation of translation in response to stress; cellular response to glucose starvation; pancreas development; regulation of fatty acid metabolic process; angiogenesis; skeletal development; protein homooligomerization; ER overload response; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; positive regulation of signal transduction; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; insulin-like growth factor receptor signaling pathway; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis