DCLK1 sirna

DCLK1 siRNA (Rat)

Cat. Num. AAA8232633

• Gene Names: Dclk1; Ania4; Cpg16; Dcamkl1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DCLK1; DCLK1 siRNA (Rat); CPG16; DCAMKL1; Serine/threonine-protein kinase DCLK1; Calcium/calmodulin-dependent protein kinase type I-like CPG16; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1; DCLK1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DCLK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 433

• Applicable Applications for DCLK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DCLK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DCLK1 sirna

siRNA to inhibit DCLK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17985955
• NCBI GeneID: 83825
• NCBI Accession #: NP_445795.1
• NCBI GenBank Nucleotide #: NM_053343.3
• UniProt Accession #: O08875
• Molecular Weight: 47,681 Da
• NCBI Official Full Name: serine/threonine-protein kinase DCLK1 isoform 1
• NCBI Official Synonym Full Names: doublecortin-like kinase 1
• NCBI Official Symbol: Dclk1
• NCBI Official Synonym Symbols: Ania4; Cpg16; Dcamkl1
• NCBI Protein Information: serine/threonine-protein kinase DCLK1
• UniProt Protein Name: Serine/threonine-protein kinase DCLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Dclk1
• UniProt Synonym Gene Names: Cpg16; Dcamkl1
• UniProt Entry Name: DCLK1_RAT

NCBI Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. This gene is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.[provided by RefSeq, Sep 2010]

Uniprot Description

DCAMKL1: a serine-threonine kinase of the CAMK family. May be involved in a calcium-signaling pathway controling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. Genetic and physical interactions with Doublecortin, which causes lissencephaly. A putative marker of quiescent stem cells. Four alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Nuclear receptor co-regulator; Protein kinase, CAMK; CAMK group; DCAMKL family

Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity

Biological Process: axonogenesis; axon extension; dendrite morphogenesis; response to virus; forebrain development; neuron migration; central nervous system projection neuron axonogenesis; brain development; protein amino acid phosphorylation