Western Blot (WB)
(Figure 1. Western blot analysis of ETF1 using anti-ETF1 antibody.)
Rabbit eRF1/ETF1 Polyclonal Antibody | anti-ETF1 antibody
Anti-eRF1/ETF1 Antibody
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
eRF1/ETF1; Polyclonal Antibody; Anti-eRF1/ETF1 Antibody; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Erf1; Protein Cl1; TB3-1; ETF1; ERF1; RF1; SUP45L1; anti-ETF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
437
Applicable Applications for anti-ETF1 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Human, Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of ETF1 using anti-ETF1 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of ETF1 using anti-ETF1 antibody.)
Related Product Information for anti-ETF1 antibody
Description: Rabbit IgG polyclonal antibody for eRF1/ETF1 detection. Tested with WB, Direct ELISA in Human; Mouse; Rat.
Background: Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Background: Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
References
1. Frolova, L., Le Goff, X., Rasmussen, H. H., Cheperegin, S., Drugeon, G., Kress, M., Arman, I., Haenni, A.-L., Celis, J. E., Philippe, M., Justesen, J., Kisselev, L. A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor. Nature 372: 701-703, 1994.
2. Guenet, L., Henry, C., Toutain, B., Dubourg, C., Le Gall, J. Y., David, V., Le Treut, A. Eukaryotic translation termination factor gene (ETF1/eRF1) maps at D5S500 in a commonly deleted region of chromosome 5q31 in malignant myeloid diseases. Cytogenet. Cell Genet. 88: 82-86, 2000.
3. Hansen, L. L., Jakobsen, C. G., Justesen, J. Assignment of the human translation termination factor 1 (ETF1) to 5q31.1 and of the proximal marker D5S1995 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 256-257, 1999.
2. Guenet, L., Henry, C., Toutain, B., Dubourg, C., Le Gall, J. Y., David, V., Le Treut, A. Eukaryotic translation termination factor gene (ETF1/eRF1) maps at D5S500 in a commonly deleted region of chromosome 5q31 in malignant myeloid diseases. Cytogenet. Cell Genet. 88: 82-86, 2000.
3. Hansen, L. L., Jakobsen, C. G., Justesen, J. Assignment of the human translation termination factor 1 (ETF1) to 5q31.1 and of the proximal marker D5S1995 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 256-257, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eukaryotic peptide chain release factor subunit 1 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation termination factor 1
NCBI Official Symbol
ETF1
NCBI Official Synonym Symbols
ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
NCBI Protein Information
eukaryotic peptide chain release factor subunit 1
UniProt Protein Name
Eukaryotic peptide chain release factor subunit 1
Protein Family
UniProt Gene Name
ETF1
UniProt Synonym Gene Names
ERF1; RF1; SUP45L1; Eukaryotic release factor 1; eRF1
UniProt Entry Name
ERF1_HUMAN
NCBI Description
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013]
Research Articles on ETF1
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Product Notes
The ETF1 etf1 (Catalog #AAA1753102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-eRF1/ETF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eRF1/ETF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Human, Mouse, Rat) Direct ELISA: 0.1-0.5ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the ETF1 etf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eRF1/ETF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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