CTSC sirna

CTSC siRNA (Rat)

Cat. Num. AAA8208181

• Gene Names: Ctsc; CATC; DPPI; DPP-I
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSC; CTSC siRNA (Rat); Dipeptidyl peptidase 1; Cathepsin C; Cathepsin J; Dipeptidyl peptidase I; DPP-I; DPPI; Dipeptidyl transferase; CTSC sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CTSC siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 462

• Applicable Applications for CTSC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CTSC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSC sirna

siRNA to inhibit CTSC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393218
• NCBI GeneID: 25423
• NCBI Accession #: NP_058793.1
• NCBI GenBank Nucleotide #: NM_017097.1
• UniProt Accession #: P80067; P80068
• Molecular Weight: 52,235 Da
• NCBI Official Full Name: dipeptidyl peptidase 1
• NCBI Official Synonym Full Names: cathepsin C
• NCBI Official Symbol: Ctsc
• NCBI Official Synonym Symbols: CATC; DPPI; DPP-I
• NCBI Protein Information: dipeptidyl peptidase 1
• UniProt Protein Name: Dipeptidyl peptidase 1
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: Ctsc
• UniProt Synonym Gene Names: DPP-I; DPPI
• UniProt Entry Name: CATC_RAT

NCBI Description

acts as a cysteine dipeptidyl aminopeptidase; may play a role in immune and inflammatory response [RGD, Feb 2006]

Uniprot Description

CTSC: Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B. Can also activate neuraminidase and factor XIII. Defects in CTSC are a cause of Papillon-Lefevre syndrome (PLS); also known as keratosis palmoplantaris with periodontopathia. PLS is an autosomal recessive disorder characterized by palmoplantar keratosis and severe periodontitis affecting deciduous and permanent dentitions and resulting in premature tooth loss. The palmoplantar keratotic phenotype vary from mild psoriasiform scaly skin to overt hyperkeratosis. Keratosis also affects other sites such as elbows and knees. Defects in CTSC are a cause of Haim-Munk syndrome (HMS); also known as keratosis palmoplantaris with periodontopathia and onychogryposis or Cochin Jewish disorder. HMS is an autosomal recessive disorder characterized by palmoplantar keratosis, onychogryphosis and periodontitis. Additional features are pes planus, arachnodactyly, and acroosteolysis. Defects in CTSC are a cause of aggressive periodontititis type 1 (AP1); also known as juvenile periodontitis (JPD) and prepubertal periodontitis (PPP). AP1 is characterized by severe and protracted gingival infections, leading to tooth loss. AP1 inheritance is autosomal dominant. Belongs to the peptidase C1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; EC 3.4.14.1; Protease

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; lysosome

Molecular Function: identical protein binding; protein self-association; serine-type endopeptidase activity; cysteine-type endopeptidase activity; chaperone binding; apoptotic protease activator activity; chloride ion binding; phosphatase binding; cysteine-type peptidase activity

Biological Process: response to organic substance; apoptosis; proteolysis; T cell mediated cytotoxicity; aging