CRHR1 sirna

CRHR1 siRNA (Rat)

Cat. Num. AAA8220977

• Gene Names: Crhr1; CRFR1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRHR1; CRHR1 siRNA (Rat); CRHR; Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1; CRHR1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CRHR1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 415

• Applicable Applications for CRHR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CRHR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRHR1 sirna

siRNA to inhibit CRHR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13591892
• NCBI GeneID: 58959
• NCBI Accession #: NP_112261.1
• NCBI GenBank Nucleotide #: NM_030999.4
• UniProt Accession #: P35353
• Molecular Weight: 47,842 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 1 isoform 1
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: Crhr1
• NCBI Official Synonym Symbols: CRFR1
• NCBI Protein Information: corticotropin-releasing factor receptor 1
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• Protein Family: Corticotropin-releasing factor receptor
• UniProt Gene Name: Crhr1
• UniProt Synonym Gene Names: Crhr; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_RAT

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. The encoded protein is a receptor for corticotropin-releasing factor (CRH) and urocortin (UCN). The interaction of this protein with CRH and UCN triggers G-protein coupled signaling. This protein plays a pivotal role in mediating the CRH-elicited effects in depression and anxiety. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass

Cellular Component: multivesicular body; cell soma; membrane; apical part of cell; cell; dendrite; integral to membrane; dendritic spine; plasma membrane; trans-Golgi network; vesicle; intrinsic to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity; peptide binding

Biological Process: fear response; hypothalamus development; hormone-mediated signaling; adrenal gland development; regulation of synaptic plasticity; positive regulation of mast cell degranulation; behavioral response to ethanol; G-protein signaling, adenylate cyclase activating pathway; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; behavioral response to pain; general adaptation syndrome, behavioral process; epithelial cell differentiation; neuropeptide signaling pathway; adrenocorticotropin hormone secretion; behavioral response to cocaine; positive regulation of cAMP biosynthetic process; negative regulation of epinephrine secretion; response to hypoxia; visual learning; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; response to electrical stimulus