CAMK2G sirna

CAMK2G siRNA (Rat)

Cat. Num. AAA829250

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK2G; CAMK2G siRNA (Rat); Calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CaMK-II subunit gamma; CAMK2G sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CAMK2G siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 527

• Applicable Applications for CAMK2G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CAMK2G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK2G sirna

siRNA to inhibit CAMK2G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19424316
• NCBI GeneID: 171140
• NCBI Accession #: NP_598289.1
• NCBI GenBank Nucleotide #: NM_133605.1
• UniProt Accession #: P11730; Q64003; Q64004
• Molecular Weight: 55,961 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit gamma
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II gamma
• NCBI Official Symbol: Camk2g
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit gamma
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit gamma
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2g
• UniProt Synonym Gene Names: CaM kinase II subunit gamma; CaMK-II subunit gamma
• UniProt Entry Name: KCC2G_RAT

NCBI Description

gamma chain of the CaM kinase II; involved in regulation of potassium currents, particularly in response to oxidative stress [RGD, Feb 2006]

Uniprot Description

CAMK2G: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Kinase, protein; CAMK group; CAMK2 family

Cellular Component: sarcoplasmic reticulum membrane; membrane

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein homodimerization activity; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: regulation of long-term neuronal synaptic plasticity; nervous system development; calcium ion transport; protein amino acid autophosphorylation; response to hypoxia; response to oxidative stress; cell differentiation; protein amino acid phosphorylation; protein oligomerization; G1/S transition of mitotic cell cycle