CALR sirna

CALR siRNA (Rat)

Cat. Num. AAA8211508

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CALR; CALR siRNA (Rat); Calreticulin; CALBP; CRP55; Calcium-binding protein 3; CABP3; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; CALR sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CALR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 416

• Applicable Applications for CALR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CALR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CALR sirna

siRNA to inhibit CALR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11693172
• NCBI GeneID: 64202
• NCBI Accession #: NP_071794.1
• NCBI GenBank Nucleotide #: NM_022399.2
• UniProt Accession #: P18418; P10452
• Molecular Weight: 47,995 Da
• NCBI Official Full Name: calreticulin
• NCBI Official Synonym Full Names: calreticulin
• NCBI Official Symbol: Calr
• NCBI Protein Information: calreticulin
• UniProt Protein Name: Calreticulin
• Protein Family: Calretinin
• UniProt Gene Name: Calr
• UniProt Synonym Gene Names: CABP3; ERp60
• UniProt Entry Name: CALR_RAT

NCBI Description

Ca(2+)-binding molecular chaperone that promotes folding of proteins carrying N-linked glycans [RGD, Feb 2006]

Uniprot Description

Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Calcium-binding

Cellular Component: Golgi apparatus; extracellular space; protein complex; cell surface; focal adhesion; sarcoplasmic reticulum lumen; smooth endoplasmic reticulum; intracellular membrane-bound organelle; sarcoplasmic reticulum; endoplasmic reticulum; endoplasmic reticulum lumen; acrosome; cytosol; extracellular matrix; polysome; membrane; perinuclear region of cytoplasm; cytoplasm; nucleus; external side of plasma membrane

Molecular Function: integrin binding; mRNA binding; protein binding; hormone binding; androgen receptor binding; iron ion binding; ubiquitin protein ligase binding; unfolded protein binding; calcium ion binding; carbohydrate binding; glycoprotein binding; peptide binding

Biological Process: response to drug; negative regulation of steroid hormone receptor signaling pathway; protein folding; protein stabilization; regulation of meiosis; positive regulation of cell cycle; response to testosterone stimulus; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; response to estradiol stimulus; peptide antigen assembly with MHC class I protein complex; response to organic substance; negative regulation of retinoic acid receptor signaling pathway; negative regulation of neuron differentiation; positive regulation of phagocytosis; negative regulation of translation; positive regulation of cell proliferation; spermatogenesis; protein export from nucleus; cortical actin cytoskeleton organization and biogenesis; cell cycle arrest; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication