BIRC5 sirna

BIRC5 siRNA (Rat)

Cat. Num. AAA8220917

• Gene Names: Birc5; AP14
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BIRC5; BIRC5 siRNA (Rat); Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; BIRC5 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BIRC5 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 142

• Applicable Applications for BIRC5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BIRC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BIRC5 sirna

siRNA to inhibit BIRC5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11560097
• NCBI GeneID: 64041
• NCBI Accession #: NP_071610.1
• NCBI GenBank Nucleotide #: NM_022274.1
• UniProt Accession #: Q9JHY7
• Molecular Weight: 16,692 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 5
• NCBI Official Synonym Full Names: baculoviral IAP repeat-containing 5
• NCBI Official Symbol: Birc5
• NCBI Official Synonym Symbols: AP14
• NCBI Protein Information: baculoviral IAP repeat-containing protein 5
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 5
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: Birc5
• UniProt Entry Name: BIRC5_RAT

NCBI Description

functions as an inhibitor of programmed cell death [RGD, Feb 2006]

Uniprot Description

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Cellular Component: centriole; nuclear chromosome; spindle microtubule; cytoplasmic microtubule; apical plasma membrane; cytoplasm; midbody; cytosol; nucleus; interphase microtubule organizing center; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; enzyme binding; zinc ion binding; protein heterodimerization activity; microtubule binding; chaperone binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; cofactor binding; Ran GTPase binding

Biological Process: response to drug; response to peptide hormone stimulus; transcription, DNA-dependent; regulation of mitotic cell cycle; response to glucocorticoid stimulus; establishment of chromosome localization; regulation of signal transduction; protein ubiquitination; positive regulation of cell cycle; negative regulation of caspase activity; microtubule cytoskeleton organization and biogenesis; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; positive regulation of exit from mitosis; negative regulation of apoptosis