Western Blot (WB)
(UBE2U Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UBE2U antibody detected the UBE2U protein (arrow).)
Rabbit anti-Human UBE2U Polyclonal Antibody | anti-UBE2U antibody
UBE2U, NT (UBE2U, Ubiquitin-conjugating enzyme E2 U, Ubiquitin carrier protein U, Ubiquitin-protein ligase U) (PE)
Gene Names
UBE2U; RP4-636O23.1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
UBE2U; Polyclonal Antibody; NT (UBE2U; Ubiquitin-conjugating enzyme E2 U; Ubiquitin carrier protein U; Ubiquitin-protein ligase U) (PE); Ubiquitin-protein ligase U; anti-UBE2U antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-UBE2U antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
UBE2U antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2U.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(UBE2U Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UBE2U antibody detected the UBE2U protein (arrow).)
Western Blot (WB)
(UBE2U Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UBE2U antibody detected the UBE2U protein (arrow).)
Related Product Information for anti-UBE2U antibody
UBE2U catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
Product Categories/Family for anti-UBE2U antibody
References
Markson, G., et al. Genome Res. 19(10):1905-1911(2009). van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~52 kDa
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2U (putative) (UBE2U), mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2U (putative)
NCBI Official Symbol
UBE2U
NCBI Official Synonym Symbols
RP4-636O23.1
NCBI Protein Information
ubiquitin-conjugating enzyme E2 U; ubiquitin-protein ligase U; ubiquitin carrier protein U
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 U
Protein Family
UniProt Gene Name
UBE2U
UniProt Entry Name
UBE2U_HUMAN
Uniprot Description
Function: Catalyzes the covalent attachment of ubiquitin to other proteins
By similarity.
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway: Protein modification; protein ubiquitination.
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Similar Products
Product Notes
The UBE2U ube2u (Catalog #AAA6360826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2U, NT (UBE2U, Ubiquitin-conjugating enzyme E2 U, Ubiquitin carrier protein U, Ubiquitin-protein ligase U) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2U can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UBE2U ube2u for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2U, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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